The zinc transporter ZIP10 regulates epidermal morphogenesis

We performed an mRNA-sequencing experiment using ZIP10 positive and negative cells isolated from the ventral skins of WT mice and analyzed gene expression profiles of those cells to identify the functional differences between the two cell types. ZIP10 positive and negative cells from the ventral skins of one-week-old WT mice were sorted by flow cytometry. From those cells, we isolated mRNAs and analyzed gene expression profiles by mRNA sequencing.我们针对从野生型（Wild Type, WT）小鼠腹部皮肤分离得到的ZIP10阳性与ZIP10阴性细胞开展了mRNA测序（mRNA-sequencing）实验，并对这些细胞的基因表达谱进行分析，以明确两种细胞类型间的功能差异。本研究通过流式细胞术分选出一周龄野生型（WT）小鼠腹部皮肤中的ZIP10阳性与阴性细胞。随后我们从上述细胞中分离mRNA，并通过mRNA测序技术分析其基因表达谱。