Genetic diversity and efficacy of natural selection in spiders with pre-copulatory sexual cannibalism

Factors that increase reproductive variance among individuals act to reduce effective population size (Ne), which accelerates loss of genetic diversity and decreases efficacy of purifying selection. These factors include sexual cannibalism, offspring investment, and mating system. Pre-copulatory sexual cannibalism where the female consumes the male prior to mating exacerbates this effect. We performed comparative transcriptomics in two spider species, the cannibalistic Trechaleoides biocellata and the non-cannibalistic T. keyserlingi, to generate genomic evidence to support these predictions. First, we estimated heterozygosity and found that genetic diversity is relatively lower in the cannibalistic species. Second, we calculated dN/dS ratios as a measure of purifying selection, higher dN/dS ratio indicated relaxed purifying selection in the cannibalistic species. These results are consistent with the hypothesis that sexual cannibalism impacts operational sex ratio and demographic proce..., Spider collections and RNA extractions
We collected six adult males from each species (T. keyserlingi and T. biocellata), consisting of two males from three populations where the two species co-occur in Uruguay 1) Parque San Miguel (33°41′51″S 53°32′00″W) Rocha; 2) Quebrada de los Cuervos (32º55’39’’S 54º27’25’’W), Treinta y tres and 3) Paso Centurión (32°8′2″S 53°47′55″W) Cerro Largo. We additionally collected two males from Paratrechalea ornata, from Quebrada de los Cuervos, as an outgroup. We chose only males to avoid any biases related to possible sex differences in gene expression. Once in the laboratory, we flash froze them in liquid nitrogen and stored them in -80ºC until RNA extraction. We extracted RNA from half of the cephalothorax using RNAasy Kit–QIAGEN following the manufacturer’s recommendations. Then, we stabilized the extractions in an ethanol (EtOH) precipitation. Library preparation (TruSeq Stranded mRNA LT Sample Prep Kit) and sequencing (PE, 101bp, Illumina platform)..., , # Genetic diversity and efficacy of natural selection in spiders with pre-copulatory sexual cannibalism

## Decription of the data and file structure

**dN.txt** : Non-synonymous rates estimates.
dNP: non-synonymous rates *Paratrechalea ornata* 
dNK: non-synonymous rates *Trechaleoides Keyserlingi* 
dNB: non-synonymous rates *Trechaleoides biocellata* 

**dNdS.txt** : Non-synonymous to Synonymous ratio estimates.
wP: Non-synonymous to Synonymous ratio estimates for *Paratrechalea ornata* 
wK: Non-synonymous to Synonymous ratio estimates for *Trechaleoides Keyserlingi*
wB: Non-synonymous to Synonymous ratio estimates for *Trechaleoides biocellata* 

**dS.txt** : Synonymous rates estimates. 
dSP: Synonymous rates *Paratrechalea ornata*
dSK: Synonymous rates *Trechaleoides Keyserlingi*
dSB: Synonymous rates *Trechaleoides biocellata*.

**He.csv** : Heterozygosity estimates.
Spp: species, Pop: populations
Ind: individuals, Totbp: total base pairs
Totsnp: total single nucleotide polymorphism...