Marcks and Marcks-like 1 proteins promote spinal cord development and regeneration in Xenopus

Marcks and Marcksl1 are abundant proteins that shuttle between the cytoplasm and membrane to modulate multiple cellular processes, including cytoskeletal dynamics, proliferation, and secretion. Here, we performed loss- and gain-of-function experiments in Xenopus laevis to reveal the novel roles of these proteins in spinal cord development and regeneration. We show that Marcks and Marcksl1 have partly redundant functions and are required for normal neurite formation and proliferation of neuro-glial progenitors during embryonic spinal cord development and for its regeneration during tadpole stages. Rescue experiments in Marcks and Marcksl1 loss of function animals further suggested that some of the functions of Marcks and Marcksl1 in the spinal cord are mediated by phospholipid signaling. Taken together, these findings identify Marcks and Marcksl1 as critical new players in spinal cord development and regeneration and suggest new pathways to be targeted for therapeutic stimulation of spin..., Images have been collected using fluorescent and confocal microscopy. Fluorescence intensity was measured using Image J and statistically analysed in GraphPad Prism. , , # Marcks and Marcks-like 1 proteins promote spinal cord development and regeneration in Xenopus

[https://doi.org/10.5061/dryad.b2rbnzsqm](https://doi.org/10.5061/dryad.b2rbnzsqm)

## Description of the data and file structure

In this dataset you will find folders for each **Main Figure** and corresponding **Supplementary Figure** in El Amri, M., Pandit, A., & Schlosser, G. (2024).

This repository contains raw images, corresponding data points used for quantification and statistical analysis, Sanger sequencing data, and swimming distance values used to create the figures of the associated publication, with each file representing the data for one figure panel named accordingly with the publication.

Unmerged immunohistochemical and in-situ hybridisation images were obtained by Fluorescent or Confocal microscopy (.jpg or .tiff). Cell counts or fluorescence intensity were quantified using Image J, averaged and plugged in GraphPad Prism for statistical analysis (.pzfx). Sequencing res...