Genome sequencing of fission yeast strains.. Schizosaccharomyces pombe

The fission yeast Schizosaccharomyces pombe is an ideal model organism for studying epigenetics. As S. pombe typically grows as haploid cells and possesses a small genome (12.8 Mb), novel genetic variations corresponding to mutant phenotypes can be easily identified by whole genome sequencing. In this project, we first sequenced the genomes of a wild-type strain and a mutant strain possessing the rpb2-m203 mutation to confirm the ability of our pipeline to identify single nucleotide polymorphisms. Next, we sequenced a strain with no apparent phenotype that survived three rounds of UV irradiation to check the effect of UV irradiation. In a genetic screen, we isolated a silencing mutant named K20. The genome of the original isolate of K20 was sequenced to identify corresponding mutation.