Comparison of the Helios narrow bore and wide bore injectors to the CyTOF2 on PBMC samples

To evaluate the staining quality of parallel PBMC aliquots of the same sample acquired on the same Helios instrument using the original narrow bore (NB) injector and the modified wide bore (WB) injector using both H20 and Fluidigm's CAS buffer during acquisition.
Conclusion:
Using the WB injector in conjunction with Fluidigm's CAS buffer during acquisition improves data quality collected on the Helios. When comparing the 3 configurations (NB+H20, WB+H20 and WB+CAS), the WB+CAS configuration improves data quality compared to the original NB injector. Marker medians and CVs obtained using the WB+CAS acquisition protocol were comparable or superior to the CyTOF2. The same stained PBMC sample was divided into multiple aliquots for acquisition on the CyTOF2 and the three Helios configurations (NB+H20, WB+H20, WB+CAS).