Arabidopsis thaliana Hi-C

Expression of eukaryotic protein-coding genes is regulated by multiple protein binding DNA sequences, generally referred as “transcription control region”. This locus control region may be situated near the target genes or very far. We focused on identification of long range regulatory elements using Hi-C which is a powerful method to capture the DNA elements that physically interact with other region of chromosome in genome wide fashion. We have sequenced 6 different Hi-C libraries through HiSeq 2500 and identified the thousands of long range chromatin interaction in Arabidopsis thaliana in controlled condition (wild-type) as well as in biotic (salicylic acid treatment) and abiotic stress (heat treatment). RNA-Seq correspond to the same Hi-C libraries were also perform for correlation among the interaction and gene expression. To understand the biological significance of these chromatin interactions, we have annotated these interactions and correlated them with the transcriptome data. To understand the effect of epigenetic states on chromosomal organization, we correlate the interaction data with different epigenetic landscape of Arabidopsis. Thus our study provide global mapping of functional chromatin interactions in genome wide fashion which likely to uncover the nuclear organization and chromosomal architecture of genome and its regulation.