Metatranscriptomic analysis for marine RNA virome using FLDS sequecing method

The recent updates of the diversity of RNA viromes are generally based only on RdRP sequence whereas complete genomes are necessary to infer with confidence virus replication and expression strategies, and to elucidate evolutionary relationships. To overcome this problem, we developed a dsRNA-derived cDNA construction and sequencing method, FLDS (Fragmented and primer Ligated DsRNA Sequencing), that enabled us to determine complete sequences of intracellular long dsRNA molecules. Using this method, we sequenced dsRNA metatranscriptomic libraries from North Pacific and Indian Oceans.