A Controllable Photoresponsive Potassium Transporter

<b>Artificial photoresponsive transport systems are particularly intriguing because they offer the potential for precise spatio-temporal control, rapid response times and minimal toxicity associated with the light. We report here a reusable, photoresponsive, ion-transporting molecular rocket, achieving reusability through a photoregulated β-cyclodextrin (β-CD)-azobenzene host-guest complex. In this complex, a lipid-anchored β-CD serves as the rocket launch base, tightly immobilizing through host-guest interactions the otherwise freely moving trans-rocket derived from a crown ether-modified trans-azobenzene motif, but rapidly releasing most of it upon 365 nm UV irradiation that converts trans-rocket to cis-rocket. The launched cis-rocket functions well as a highly efficient potassium transporter, delivering a low EC50 value of 1.51 μM and a drastic transport activity enhancement by ~ 400% relative to the base-bound trans-rocket (EC50 = 7.46 μM), with on-off activities repeatedly regulated by light. In the absence of the base, the on-off activities between cis- and trans-rockets however differ by only 80% (1.35 μM vs 2.45 μM). </b>