Divergences of sperm DNA methylomes between monozygotic twin seed bulls reveal fertility-related genes (RNA-Seq)

Semen quality is extremely important for fertility, and it is easily influenced by environmental factors and can induce subfertility of the next generations. The sperm DNA methylomes of the MZ twin bulls were investigated by WGBS at single-base resolution. We detected DMRs between the twin bulls with divergent sperm qualities. We also conducted transcriptome sequencing for the MZ twin bulls in three replicates. We investigated the relationship between methylation and gene expression. Comparison of sperm transcriptome profiles between monozygous twin bulls with divergent male fertility.