Gene expression in the GWAT of ob/ob and ob/ob/Fsp27-/- mice

GWAT store most of the TAG in mice, ob/ob mice is an obese mice. Ob/ob/Fsp27-/- mice are lean when compared with ob/ob mice. The GWAT weight was dramatically reduced in ob/ob/Fsp27-/- mice. We next extract the total RNA from the GWAT of ob/ob and ob/ob/Fsp27-/- mice, to perform microaary analysis using Mouse Genome 430 2.0 arrays, Affymetrix. We then analysised the up-regulated and down regulated pathways. We extract the RNA of GWAT from 4 month old mice and hybridization on Affymetrix microarrays. We then analysis the data.GWAT（性腺白色脂肪组织，gonadal white adipose tissue）在小鼠体内储存大部分三酰甘油（TAG，triacylglycerol）。ob/ob小鼠为肥胖型小鼠。与ob/ob小鼠相比，Ob/ob/Fsp27-/-小鼠体型偏瘦。Ob/ob/Fsp27-/-小鼠的GWAT重量显著降低。随后我们从ob/ob与Ob/ob/Fsp27-/-小鼠的GWAT中提取总RNA，采用Affymetrix公司的Mouse Genome 430 2.0芯片进行基因芯片（microarray）分析。随后我们对上调通路与下调通路进行分析。我们从4月龄小鼠的GWAT中提取RNA，并将其与Affymetrix基因芯片进行杂交。随后我们对所得数据进行分析。