Tissue resident memory CD4+ T cells after neonatal or adult HMPV infection

Tissue resident memory cells are long-lived sentinels that are generated after exposures at mucosal sites. In this experiment, neonatal (day of life 4-6) or adult (6-8 week old) mice were infected with human metapneumovirus (HMPV). 5 weeks following infection, tissue resident cells were identified using i.v. exclusion of circulating cells and flow sorted. Cells were then multiplexed and processed using the 10X genomics platform. Overall design: Experimental conditions include: NeoTRMs (TRMs isolated from the lung 5 weeks after neonatal mouse infection with HMPV) or AdultTRMs (TRMs isolated from the lung 5 weeks after adult mouse infection with HMPV)