GATA3 truncation mutants alter EMT related gene expression via partial motif recognition in luminal breast cancer cell

GATA3 is one of the most frequently mutated genes in breast cancer. The roles of GATA3 mutations remain elusive. In this study, we characterized newly established luminal breast cancer cell lines that stably express different GATA3 patient-derived mutants (Splice site deletion, C321 frame-shift, and A333 frame-shift mutants). The expression of these mutants lead to altered expression of EMT related genes as well as redistribution of GATA3. Genome-wide mapping of chromatin localization of GATA3 and transcriptome analysis in GATA3 mutant cells