Stabilization of human whole blood samples for multi-center and retrospective immunophenotyping studies - Flow Cytometry, Panel 1

This .fcs files form the part of the manuscript titled “Stabilization of human whole blood samples for multi-center and retrospective immunophenotyping studies”. The .fcs files presented in this experiment were obtained for flow cytometry (FC) part of the manuscript. Herein we have evaluated two whole blood preservation protocols that allow rapid sample processing and long-term stability for flow cytometry experiments. Two fixation buffers were used, Phosphoflow Fix and Lyse (BD) and Proteomic Stabilizer (PROT) to fix and freeze whole blood samples for up to 6 months. Fixed samples were compared to the fresh blood.
Conclusion:
In summary, we obtained good correlation when comparing fresh and frozen samples for both buffers. However, fixation affected the detection of CD16-PC7 and CD56-PC5.5 and introduced changes in light scattering properties. Once preserved, the samples were stable for at least 6 months at -80°C.
Notes:
Posted .fcs files contain raw data. Data were analyzed with FlowJo software as indicated in the methods. Both Panel 1 stained and unstained samples are provided. The gating strategy was performed as described in the figure 1 in the manuscript. FACSVerse cytometer (BD Biosciences) was previously calibrated with Rainbow 8-peak beads.