Recent breakthroughs with synthetic budding yeast chromosomes expedite the creation of synthetic mammalian chromosomes and genomes. Mammals, unlike budding yeast, depend on the histone H3 variant, CEN
Whether synthetic genomescan power life has attracted broad interest in the synthetic biology field, especially when the synthetic genomes are extensively modified with thousands of designer features.
Data accompanying the paper "Pooled clone collections by multiplexed CRISPR-Cas12a-assisted gene tagging in yeast" by Buchmuller and Herbst et al, 2019, Nat Communications. This contains raw NGS data