Microarray profiling of total testicular transcriptome in mouse

Gene expression during spermatogenesis is highly variable and this differential pattern is very important for the successive culmination of different stages of the process, leading to production of the male gamete. Taking the time window of first wave of spermatogenesis, we did a microarray profiling of total testicular transcriptome in mouse and found several significant patterns of variable gene expression, forming upregulated and downregulated clusters among the three stages analyzed here. Mouse testis tissue were taken at three stages, 8 days, 16 days and 24 days, during first wave of spermatogenesis for RNA extraction and hybridization on the Affymetrix microarrays. Three biological replicates per age group were included and comparisons were done as 16 days vs 8 days, 24 days vs 16 days and 24 days vs 8 days testis transcriptome profile.精子发生（spermatogenesis）过程中的基因表达呈现高度异质性，该差异表达模式对于该过程不同阶段的依次完成至关重要，最终实现雄性配子的生成。本研究聚焦小鼠精子发生首波的时间窗口，对小鼠全睾丸转录组（transcriptome）开展微阵列谱分析（microarray profiling），鉴定出多组显著的差异基因表达模式，并在所分析的三个阶段中划分得到上调基因簇与下调基因簇。本研究在精子发生首波阶段的三个时间点——8日龄、16日龄及24日龄——采集小鼠睾丸组织，用于RNA提取并在Affymetrix微阵列上完成杂交实验。每个年龄组设置3次生物学重复（biological replicates），并分别针对16日龄与8日龄、24日龄与16日龄以及24日龄与8日龄的小鼠睾丸转录组谱开展对比分析。