Proteomic analysis of cooperating yeast metabolic communities during ageing

Yeast (Saccharomyces cerevisiae) cooperating metabolic communities (SeMeCos) were chronologically aged in minimal media (no amino acid supplementation). Samples were collected during exponential, early stationary and stationary phases for proteomic analysis. Comparison was performed between wild-type (knock-in, "kin"), 4p-SeMeCo ("4p") and MET15-3p-SeMeCo ("3p") cultures. Four independent biological replicates were acquired for each timepoint measured.