Using saccharomyces cerevisiae ATCC26603 as the parental strain, the xylose-metabolizing strain CRD5HS was constructed. Adaptive laboratory evolution of CRD5HS for ethanol tolerance were conducted, an
DNA-seq of yeast tetrads, ChIP-seq and MNase-seq, used for comparing the number of crossover generated per meiosis, DSB intensity and histone occupation between diploid and tetraploid yeast.
We developed a CRISPR-Cas assisted random mutation (CARM) technology for whole genome mutagenesis. As a proof-of-principle, CARM was applied to evolve the capacity of Saccharomyces cerevisiae to produ