Daniela Cimini, Lisa A Cameron, Edward D Salmon (2011) CIL:12018, Potorous tridactylus, epithelial cell. CIL. Dataset

Metaphase PtK1 cell microinjected with X-rhodamine-labeled tubulin and Alexa 488-labeled CENP-F antibodies to fluorescently label kinetochore fibers (red) and kinetochores/spindle poles (green), respectively. The movie shows that sister kinetochore pairs oscillate between the two spindle poles as their respective kinetochore fibers lengthen and shorten.

Digital images were collected with an Orca ER cooled CCD camera (Hamamatsu Photonics) coupled to a Yokogawa spinning disk confocal CS10 unit (Perkin Elmer Life Sciences Wallac), which was attached to a Nikon TE300 inverted microscope. Illumination was achieved via an Argon/Krypton laser and 488 nm and 568 nm filters in an excitation filter wheel (Sutter Instruments) through a 60 x 1.4 NA Plan-Apochromatic DIC objective lens. Nearly simultaneous fluorescence images were acquired at 488 and 568 nm at a single focal plane every 21 seconds. The movie is played at 10 frames per second. Elapsed time is shown in min:sec.

本数据集描述了中期 PtK1 细胞经 X-rhodamine 标记的微管和 Alexa 488 标记的 CENP-F 抗体进行荧光标记，分别用于标记着丝粒纤维（红色）和着丝粒/纺锤体极（绿色）。影片展示了姐妹着丝粒对在各自的着丝粒纤维伸长和缩短的过程中在两个纺锤体极之间进行振荡。数字图像由 Hamamatsu Photonics 公司生产的 Orca ER 冷却型 CCD 相机采集，并与 Perkin Elmer Life Sciences Wallac 公司的 Yokogawa 振转盘共聚焦 CS10 单元连接。该单元安装在 Nikon TE300 倒置显微镜上。照明通过 Ar/Kr 激光和激发滤光轮（Sutter Instruments）中的 488 nm 和 568 nm 滤光片实现，通过 60 x 1.4 NA Plan-Apochromatic DIC 物镜进行。在单个焦平面，每隔 21 秒以 488 nm 和 568 nm 波长几乎同时获取荧光图像。影片以每秒 10 帧的速度播放。显示的时间为分钟:秒。