Human neutrophil degranulation
收藏资源简介：
To determine neutrophil degranulation by cell surface staining of CD63 (surrogate marker for azurophilic granules) and/or CD66b ( surrogate marker for specific granules). Conclusion: Upon neutrophil activation, cell surface expression of CD63 and CD66b are increased. High expression of RhoH and myosin IIA inhibitors suppress neutrophil degranulation, as reflected by reduced cell surface expression of CD63 and/or CD66b by flow cytometry. Notes: Isolated neutrophils from healthy donors and cystic fibrosis patients; Neutrophils pre-treated with cell culture medium, healthy serum and CF serum before activation; Neutrophils pre-treated with different myosin IIA inhibitors before activation; Isotype controls were used at the same concentration: APC-conjugated Mouse IgG1, κ isotype control (clone MOPC-21; BioLegend), FITC-conjugated Mouse IgM, κ isotype control (clone MM-30; BioLegend)
