Gene expression changes of tert-butyl alchol-induced in renal cortex of rats

This study is to develop a short term and highly accurate prediction method of renal carcinogenicity based on gene expression profile of rats administrated by carcinogens. We conducted 28 days-repeated dose experiments in male SD rats with tert-butyl alchol, and the gene expression profiles of renal cortex were analyzed using custom microarrays. Experimental design is the two group (control group vs. tert-butyl alchol treated group). Three biological replicates were performed for each group. Rats were humanely sacrified by bleeding under anesthesia with CO2-O2 (4:1) or isoflurane gas inhalation 24 hr after final administration.