Expression analysis of wild-type Streptococcus pneumoniae PN4595-T23 and Vp1 deletion mutant

Investigation of differentially regulated genes in deletion of Vp1 compared to the wild-type. VP1 is a signaling peptide. Its characterization is described in Cuevas et al 2019 ADD TITLE AND REF A three chip study using RNA isolated from three separate wild-type cultures of Streptococcus pneumoniae PN4595-T23 and three separate cultures of VP1 mutant strain. Cultures were harvested at mid-log phase and used for RNA isolation and cDNA preparation and hybridized onto SpSGH array with custom designed probes. The SpSGH array covers the pneumococcal pangenome and a total of 1,930 genes were relevant for PN4595-T23. The design and validation of SpSGH array is described in Kadam et al 2015 Streptococcus pneumoniae Supragenome Hybridization Arrays for Profiling of Genetic Content and Gene Expression. Curr Protoc Microbiol. 2015; 36: 9D.4.1–9D.4.20.