Effect of depleting Myo/Nog cells on the accumulation of cells immunoreactive for muscle and beaded filament proteins in anterior lens cultures.

Anterior lens tissue was incubated with the G8 mAb or complement (Comp) alone, or G8 and complement, 20 hours after plating. Cultures were double labeled with antibodies to G8 and α-SMA, MyoD, sarcomeric myosin, filensin or CP49 on the fifth day in culture. Values are the mean ± standard deviation of the number of antibody-positive cells ÷ total cells X 100. The number of cultures scored is indicated in parentheses. No significant differences were found between the percentages of G8+ or α-SMA+ cells treated with either the G8 mAb or complement alone. Significant differences were found in cultures treated with either the G8 mAb or complement alone and G8+ complement in the percentages of G8+ (0.003 and 0.0001, respectively), α-SMA+ (0.02 and 0.006, respectively), MyoD+ (0.0001) and myosin+ cells (0.0001). No significant differences were found between the percentages of filensin+ and CP49+ cells in cultures treated with G8 or G8+ complement.