RNASeq analysis of NB1691 neuroblastoma cells treated with MDM2-p53 inhibitor RG7388 alone and in combination with temozolomide

To further identify potential mechanisms involved in combining RG7388 with temozolomide, GSEA was performed and revealed that RG7388 and temozolomide act cooperatively to upregulate the p53 pathway and downregulate MYC targets, the former resulting in significantly greater induction of several apoptotic genes. Overall design: NB1691 neuroblastoma cells were treated with DMSO, RG7388 alone, temozolomide alone, or RG7388 and temozolomide in combination for 24 and 72 hours. All experiments were performed in triplicate.