Telomere-to-mitochondria signaling by ZBP1 mediates replicative crisis (RNA-Seq)

Transcriptional response to telomere shortening or dysfunction Overall design: Comparative gene expression profiling analysis of RNA-seq data for growing and crisis IMR90E6E7 (3 replicates each); growing and crisis WI38SV40LT (3 replicates each), pre-crisis IMR90E6E7 transfected with non-targeting siRNA (control) or two individual siRNAs against ZBP1 (3 replicates each), growing IMR90E6E7 expressing either a dox-inducible TRF1(DN) or dox-inducible VP16-TRF1(DN) and constitutive expression of either ZBP1(S)WT-Flag or ZBP1(S)DZa2-Flag (3 replicates each), and pre-crisis IMR90E6E7 expressing GFP-targeting gRNA (control), MAVS-targeting gRNA, or STING-targeting gRNA (2 replicates each).