An Nfic-Hedgehog Signaling Cascade Regulates Tooth Root Development

This study aimed to perform transcriptome profiling of Nfic-/- and corresponding control tooth germ at root initiation stage to identify differentially expressed for key regulators of root development. Coordination between the Hertwig's Epithelial Root Sheath (HERS) and apical papilla (AP) is crucial for proper root development process. The Hedgehog (Hh) signaling pathway and Nfic are both involved in tooth root development. mRNA profiling of apical half of tooth germ from 6 pairs of 4-day-old Nfic-/- and littermate Nfic+/- control mice were generated using Illumina Next-Seq 500