Real Sink Automatic Collection System (RSACS) validation and implementation for in vitro simulation of physiological drug clearance and release modulation

Figure 6. Real Sink Automatic Collection System (RSACS) design, implementation, and validation for in vitro simulation of physiological drug clearance. (A) Schematic overview of the RSACS platform for mimicking physiological drug clearance. The system enables high-throughput, parallel testing of multiple drug delivery platforms with automated sample collection. It leverages custom-modified 3D printer mechanics and firmware, and it is controlled via computer interface. (B) Validation of RSACS clearance capabilities, benchmarked against in vivo pharmacokinetics in rats following subcutaneous (sub-Q) injection. Equivalent drug dosing (scaled 1000-fold for HPLC detection) was replicated in RSACS to confirm physiologically relevant clearance dynamics. (C) In vitromodulation of drug release using the nanofluidic electrochemical pump tested in RSACS. Drug concentrations in the reservoir reflect actuation dynamics, with the corresponding predicted rat pharmacokinetic profile derived from model fitting, (n=1).