Characterization of morphological alterations in micropapillary adenocarcinoma of the lung

Micropapillary adenocarcinoma of the lung is a cancer with poor prognosis and characterized by the presence of tumor cells with ring-like glandular structure floating within alveolar spaces. We investigated the association between its morphological, biochemical, and immunohistochemical characteristics and malignancy using the KU-Lu-MPPt3 cell line established from a patient with micropapillary adenocarcinoma. Two subpopulations of KU-Lu-MPPt3 cells, adhesive (AD) and clumpy and suspended (CS) cells, were prepared and subjected to DNA microarray. Microarray analysis was performed to investigate the changes in gene expression due to morphological differences in KU-Lu-MPPt3 adhesive (AD) cells and KU-Lu-MPPt3 clumpy and suspended (CS) cells established from the micropapillary adenocarcinoma of the lung. In addition, PC9 which is lung adenocarcinoma cell line was added to the sample.