<p>Identification of <i>ura6</i> mutations in tetrads.</p>

Identification of the mutations in ura6 by whole genome sequencing of spores coming from the indicated crosses. The M parental strain used to cross with the four P strains is colored in blue while the symmetrical P one is colored in red. Column C indicates the position of the mutation on chromosome 3. Column D indicates the nucleotide in the wild-type sequence of the ura6+ gene that was found mutated. Column E indicates the mutated nucleotide found in the ura6 mutants. Column F indicates the amino acid in the wild-type proteins while column G shows the change found in the mutant protein. Column H is indicating the position of the mutations in the protein Ura6. (XLSX)