Identification of new microRNA-22-5p and -199a-5p/MMP1 functional interactions in stress-induced senescent human dermal fibroblasts [mRNA]

To study and correlate modulations of miRNome with modifications of transcriptome in human stress-induced senescent fibroblasts, Agilent microarrays data were compared by bioinformatics analysis with a focus on ECM components. We identified two unreported direct interactions since we highlighted a reduction in the expression of both miR-22-5p and -199a-5p in senescent fibroblasts, correlated to an increase of MMP1 expression. Gene expression in stress-induced senescent (SIS) fibroblasts was measured after 4 days of irradiation. Four independent experiments were performed for each condition (normal human fibroblasts [NHF] and SIS-fibroblasts) using different donors for each experiment. Contributor: R&D department, SILAB, France