Wolbachia pipientis is an incredibly widespread bacterial symbiont of insects, present in an estimated 25-52% of species worldwide. Wolbachia is faithfully maternally transmitted both in a laboratory
Cells were maintained in complete media (CM) or starved for 24 hours in HBSS prior to imaging. The p:s ratios and representative images are presented for: Nup54-mEGFP494(0).
Images were collected at 4-minute intervals on a DeltaVision system equipped with a with a 60x PlanApo PH3 phase-contrast, oil immersion objective (NA = 1.4). This system has a motorized x-y-z stage,
Tilt range -70 to +70 Saxton Scheme; Magnification 11500; Defocus -10 μm; total dose 7.0E+21 e-/m2. Parent item: The Initial Stages of Template-Controlled CaCO3 Formation Revealed by Cryo-TEM Supporti
