Transcriptional_alterations_in_Dph6_Ko_MEFs. Transcriptional_alterations_in_Dph6_Ko_MEFs

Data obtained from the screens associated with the Sanger mouse pipeline has identified an interesting phenotype in this line – in particular a T cell defect, which appears to represent exhaustion. This line also appears to have an reduced survival that could be linked to increased tumour incidence, in addition they have a decreased ability to clear intravenously administered tumour cells. From investigating the function of DPH6 it performs the final amidation step in dipthamide synthesis a highly conserved modification on a single histidine residue in EEF2. The function of dipthamide in EEF2 is not understood, it is targeted by various bacterial toxins causing cell cycle arrest and cell death, a suggested mechanism is in regulating translational fidelity. In order to assess the translational fidelity we plan to compare the complete proteome to the transcriptome This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/