Effect of Lactation on Proteome of Milk Fat Globule Membrane of Laoshan Dairy Goat

We have investigated MFGM proteome of goat milk at 1, 3, 30, 90, 150, and 240 d using data-independent acquisition and data-dependent acquisition quantitative proteomics approaches. The clustering and principal component analysis revealed that the protein components in goat milk was associated with specific lactation stages. Protein-protein interaction showed that albumin had more interaction with other proteins and was considered as a central node. Our results can provide a better understanding of the MFGM proteome during the lactation cycle of dairy goats and indicate changed proteins are important for the better understanding of intrinsic physiological function of goats.

本研究采用数据独立采集和数据依赖采集的定量蛋白质组学方法，对山羊乳1、3、30、90、150和240天内的MFGM（山羊乳脂肪球膜）蛋白质组进行了深入探究。聚类分析与主成分分析揭示了山羊乳中的蛋白质成分与特定泌乳阶段密切相关。蛋白质相互作用分析表明，白蛋白与其他蛋白质的相互作用更为频繁，被视为网络中的中心节点。本研究成果有助于更深入地理解山羊泌乳周期中的MFGM蛋白质组，并指出蛋白质的变化对于揭示山羊内在生理功能的本质具有重要意义。