Sox2 levels configure the WNT response of epiblast progenitors responsible for vertebrate body formation. [EXP1_ATACSeq]

In this experiment the relationship between SOX2 levels and chromatin accessibility was assayed using ATAC-seq during embryonic stem cell differentiation. A doxycycline inducible cell line (SOX2 TetON) was employed to experimentally control SOX2 expression. Overall design: Transposase accessible chromatin profiles were generated at high read depth in three experimentall conditions. Sox2 TetON ES cells differentiated in the presence or absence of 1ug/ml doxycycline were compared to differentiated WT epiblast progenitor cells.