Demonstration that qPCR verification primers exhibit exquisite specificity for NIH3T3 (mouse embryonic fibroblasts) cDNA when directly compared to HeLa (human squamous cell carcinoma) cDNA.

Average Ct (± S.D.) values were obtained via qPCR for each gene primer set listed above when exposed to either NIH3T3 or HeLa template cDNA following standard 40 cycle SYBR Green evaluation as performed on the Roche 480 LightCycler II platform in either quadruplicate (HeLa) or triplicate (NIH3T3). HeLa Ct values scored as “Not Detected” refers to either all four replicates failing to register a detectable amplicon threshold or reflects that only one replicate achieved threshold detection, which most likely reflects stochastic amplicon formation at higher Ct values (>35); further, values that surpassed a Ct of 35 were considered as “Not Detected”.