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Genome-wide tracking of DNA polymerase III in E. coli

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NIAID Data Ecosystem2026-05-01 收录
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We used steric gate variants of DNA polymerase III that allows increased amounts of ribonucleotides to be incorporated. Ribonucleotides incorporated into genomic is hydrolyzed by alkali. The generated 5-ends are subsequently mapped. We map these ribonucleotides in the entire genome and determine if there is any strand bias.

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2023-04-14
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