Next generation lineage discovery: a case study of tuberous Claytonia L.

PREMISE OF THE STUDY: Species formation is an intuitive endpoint of reproductive isolation, but circumscribing taxa that arise during speciation can be difficult because of gene flow, morphological continuity, hybridization or polyploidization, and low sequence variation among newly diverged lineages. Nonetheless, species complexes are ubiquitous and their classification is essential for understanding how diversity influences ecosystem function. METHODS: We used modern sequencing technology to identify lineages of perennial Claytonia L., and assessed correspondence between genetic lineages and morphological/ecological variation. Subsets of 18 taxa from 63 populations were used for (a) lineage discovery using network and coalescent analyses, (b) leaf shape analyses using elliptical Fourier analysis and ordination, and (c) ecological analyses (soil chemistry, climate) using ANOVA and ordination. KEY RESULTS: Samples mainly aggregated into groups representing each of the previously recog...