Abundances of Heterotrophic Bacteria, Prochlorococcus, Synnechococcus, and Picoeucaryotes during the PARAGON2 cruise

This dataset is part of the 2022 SCOPE-PARAGON II (PARticles And Growth in the Oceanic Nutricline) research expedition (KM2209 aboard the R/V Kilo Moana), a coordinated effort to characterize particle dynamics and remineralization in the upper 500 m of the North Pacific Subtropical Gyre (more info here: http://scope.soest.hawaii.edu/data/scope2022/scope2022.html). Cruise date: Aug 4 - Aug 12 2022; Cruise location: 17-24N, ~154W. The cruise tracked a high chlorophyll feature close to the Hawaiian Islands. Particle abundances of three separate chlorophyll containing populations were enumerated by autofluorescence using a B/D Influx flow cytometer: Prochlorococcus, Synechococcus and the pico-Eukaryotes. Heterotrophic bacteria were enumerated using the DNA stain SYBR Green I and subtracting the previously obtained Prochlorococcus concentration from the DNA positive cells. Particles were collected in situ and preserved following standard protocols for analysis in the lab (~6 months after collection). 1 micrometer polystyrene beads were added to all samples as reference. Timestamp is in UTC.