Caterpillar-Induced Rice Volatile (E)-β-Farnesene Impairs the Development and Survival of Chilo suppressalis Larvae by Disrupting Insect Hormone Balance. Caterpillar-Induced Rice Volatile (E)-β-Farnesene Impairs the Development and Survival of Chilo suppressalis Larvae by Disrupting Insect Hormone Balance

In this study, an artificial feeding study containing (E)-β-farnesene (0.8g/kg) with C. suppressalis larvae was conducted to investigate its physical performance, and results revealed that the average 2nd instar duration in C. suppressalis significantly increased from 4.78 days to 6.31 days after Eβf treatment (P0.05). The mortality rates of 2nd C. suppressalis larvae were increased by 21.00-fold, compared with control(P<0.001), 3rd C. suppressalis larval increased by 6.39-fold(P<0.001). Comparative transcriptome analysis also showed that multiple DEGs involved in insect hormone biosynthesis pathway, the results of hormone assay in 3rd instar larvae showed the balance between juvenile hormones and ecdysteroids was disrupted. Eβf treatment increased juvenile hormones titers, but not the ecdysteroids. The results of qPCR were consistent with RNA-seq. Taken together, Eβf impairs the development and survival of C. suppressalis larvae by disrupting insect hormone balance. Moreover, the altered pathways were associated with carbohydrates, xenobiotics and cofactors and vitamins in C. suppressalis larvae. Altogether, our findings identify Eβf impair the development and survival of C. suppressalis larvae. Additionally, our data also revealed a potential mechanism that Eβf impaired the development and survival of C. suppressalis larval by disrupting insect hormone balance. These findings may contribute to the integrated control of C. suppressalis. Overall design: Examination of 2 different transcriptome in Chilo suppressalis.