Figure 6D. Effect of Lenti-HPV-07 immunotherapy on lung T cells in C57BL/6 with metastatic TC-1 tumors

C57BL/6 mice were injected i.v. with 1.5 × 10(5) nLuc-TC-1 cells. On day 5, mice received a single i.m. injection of 1.10(9) TU of Ctrl Lenti or Lenti-HPV-07 (n = 10-11/group). At day 15 post nLuc-TC-1 i.v. instillation, groups of untreated mice (Neg Ctrl) (n = 4) or Lenti Ctrl- (n = 10) or Lenti-HPV-07- (n = 10) treated and tumor bearing mice received an i.v. injection of PE-anti-CD45 mAb, 3 min before sacrifice ("CD45 i.v."). This in vivo staining allows cytometrical distinction of lung parenchymal (CD45i.v.-) from vascular (CD45i.v.+) hematopoietic cells. Lung T cells stained for cytometric analyses.