A Microfabrication-Based Dynamic Array Cytometer

We have developed a microfabricated device for use in
parallel luminescent single-cell assays that can sort populations upon the basis of dynamic functional responses
to stimuli. This device is composed of a regular array of
noncontact single-cell traps. These traps use dielectrophoresis to stably confine cells and hold them against
disrupting fluid flows. Using quantitative modeling, we
have designed traps with a novel asymmetric extruded-quadrupole geometry. This new trap can be physically
arrayed and electrically addressed, enabling our cytometer. Situating an array of these traps in a microchannel,
we have introduced cells into the array and demonstrated
observation of fluorescent dynamic responses followed by
sorting. Such a device has potential for use in investigating
functional processes, as revealed by temporal behavior,
in large numbers of single cells.