Effects of Mosquito Saliva on Immune System of Humanized Mice

To discover the immunogenic effects of mosquito bites on humanized NSG mice as a model for the effects of mosquito bites on humans. Single color controls (using compensation beads) were used as compensation controls. Compensation was original performed manually in FACSDiva software but was later modified in FlowJo during data analysis. The flowjo modified compensation is not included in these files. Fluorescence minus one controls (FMOs) were used as gating controls. Voltages were set using single color controls and unstained controls.