Mechanomics AFM Set 02 - ECC4 vs TGBC

Entry is a part of Mechanomics collection (10.6084/m9.figshare.c.5399826) that contains materials supporting findings presented in the following manuscript:Urbanska, M., Ge, Y. et al.,De novo identification of universal cell mechanics gene signaturesBiorXiv: https://doi.org/10.1101/2021.04.26.441418; first published: 27th April 2021, last updated: 4th Nov 2024eLife: https://doi.org/10.7554/eLife.87930; first published: 23rd March 2023, last updated: 27th Dec 2024This dataset contains mechanical measurement of ECC4 and TGBC18TKB (TGBC) carcinoma cell lines conducted with with atomic force microscope (AFM).Measurements were performed using Nanowizard 4 (JPK Instruments, Germany).Tip-less cantilevers (PNP-TR-TL, nominal spring constant k = 0.08 N m−1, Nanoworld, Switzerland) decorated with a polystyrene bead of 5-µm diameter (PS-R-5.0, microParticles, Germany) each were used as the indenters.Following sub-folders are included under each sample:• Calibration - contains data acquired during cantilever calibration• Ind - indentation curves in .jpk-force format• Ind_processed-yyyy.mm.dd-hh.mm.ss - results of processing of indentation curves with JPK data processing software (JPK DP, JPK Instruments)• Osc - records of oscillatory measurements in .jpk-force format• Osc_processed-yyyy.mm.dd-hh.mm.ss - results of processing of oscillatory measurements with JPK data processing software (JPK DP, JPK Instruments)• Pics - bright-field images of cells acquired during the measurements, cantilever is located above the cell of interest