Transcription profile of infiltrating monocytes derived from 3D liver cancer cell line spheroids

To study the molecular mechanism of tumor associated macrophages (TAMs), the RNA of infiltrating monocytes derived from in vitro liver cancer spheroid model were performed. The results indicated that the cholesterol metabolism-related genes enriched in the infiltrating monocytes, which presented the M2-like TAM phenotypes. In this study, the infiltrating monocytes isolated from 3D liver cancer cell lines consisted of infiltrating monocytes in HepG2 spheroids and the infiltrating monococytes in PLC/PRF/5 spheroids. The freshly isolated monocytes from PBMCs were control. The gene expressions were performed on NanoString nCounter Human Myeloid Innate Immunity Panel.