"Dataset for ThermiQuant\u2122 VitroMini: A Compact Dual-Sided Heater\u2013Imager Platform for Quantitative Colorimetric Isothermal Nucleic"

"Objective: Maintaining precise isothermal conditions in portable nucleic acid amplification tests (NAATs) is critical for reproducible results but remains challenging with conventional single-sided thin-film heaters, which exhibit temperature gradients and strong dependence on ambient conditions. To close this gap, we engineered ThermiQuant\u2122 VitroMini, a dual-sided heater design that achieves volumetric-level temperature uniformity using thin-film heaters while preserving optical transparency for real-time colorimetric loop-mediated isothermal amplification (LAMP) analysis on microfluidic paper-based analytical devices (\u00b5PADs). Methods: The device integrates two independently regulated indium tin oxide (ITO) heaters (8 \u03a9 each) controlled by independent  proportional\u2013integral\u2013derivative (PID) algorithms. Heaters were evaluated under controlled ambient environments of 4 \u00b0C (refrigerated), 23 \u00b0C (room temperature), and 50 \u00b0C (oven). Analytical tests were performed using a colorimetric LAMP assay targeting the SARS-CoV-2 orf7ab gene on \u00b5PADs preloaded with dried LAMP reagents, with time-lapse images (30 seconds interval) analyzed via Amplimetrics\u2122 software. Results: VitroMini maintained 65 \u00b1 0.5 \u00b0C across 4 to 50 \u00b0C ambient conditions and achieved a limit of detection of 50 copies\/reaction (6.7 copies\/\u00b5L), with quantification times (Tq) linearly correlated with log10 DNA concentration. Conclusion: Dual-sided heating eliminated temperature bias, condensation artifacts, and ambient-dependent variability while preserving optical transparency for real-time LAMP quantification. Significance: ThermiQuant\u2122 VitroMini bridges the gap between benchtop volumetric heaters and portable diagnostic devices, offering a compact, low-power, and field-deployable platform for decentralized molecular diagnostics and One Health applications.  "