Genome-wide map of AR-targets in human endometrial stromal cells treated with DHT.

Polycystic ovary syndrome (PCOS) is a common gynaecological disorder, characterised by elevated circulating androgens and aberrant expression of androgen receptor (AR) in the endometrium: The objective of this study is to evaluate the targets of AR in PCOS patients. Overexpression of AR and altered endometrial signalling pathways in PCOS patients may impact on a correct decidualization response, critically affecting the events surrounding embryo implantation and causing infertility. Endometrial stromal cells isolated from 3 PCOS patients were pooled together, grown to confluency and treated with DHT for 72h. One chromatin sample (containing genetic background from 3 different patients) was immunoprecipitated with an anti-AR antibody and subsequently submitted to ChIP-Seq