Transcriptome analysis of WT and lactoferrin knockout satellite cells grown for 4 days
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https://www.ncbi.nlm.nih.gov/sra/SRP377735
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资源简介：
Our phenotypic data suggest that lactoferrin knockdown reduces satellite cell proliferative capacity. The mouse muscle tissue was digested with collagenase, and satellite cells were obtained by flow sorting. We performed transcriptome analysis of wt and lactoferrin knockout cells grown for 4 days in vitro. Overall design: We digested mouse muscle tissue by collagenase and flow-sorted Sca1 -/CD11b -/CD34 +/a7-integrin + satellite cells. After 4 days of in vitro growth, cells were harvested, added with 1 ml Trizol, snap-frozen in liquid nitrogen, and transported to GeneDenovo Biotechnology Co. Data were extracted and normalized according to the manufacturer's standard protocol.
创建时间：
2022-07-14
