Tracking cell cycling in RPE-Fucci cell cultures via DNA content profiling
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http://flowrepository.org/id/FR-FCM-Z4GQ
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资源简介:
Tracking cell cycling in RPE-Fucci cell cultures via DNA content profiling
Conclusion:
During the cumulative tracing period, a clear serum concentration-dependent increase in the proportion of cells in S and G2/M was observed.
Notes:
Flow cytometry assessments of DNA content profiles were performed at the indicated timepoints on cultures of RPE-Fucci cells treated with different FBS concentrations. Staining with the DNA dye Hoechst 33342 was performed to determine RPE-Fucci cell fractions at different stages of the cell cycle based on their DNA content. Mock cells were used to set fluorescence background thresholds.
创建时间:
2022-09-01



