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Tracking cell cycling in RPE-Fucci cell cultures via DNA content profiling

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NIAID Data Ecosystem2026-03-13 收录
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Tracking cell cycling in RPE-Fucci cell cultures via DNA content profiling Conclusion: During the cumulative tracing period, a clear serum concentration-dependent increase in the proportion of cells in S and G2/M was observed. Notes: Flow cytometry assessments of DNA content profiles were performed at the indicated timepoints on cultures of RPE-Fucci cells treated with different FBS concentrations. Staining with the DNA dye Hoechst 33342 was performed to determine RPE-Fucci cell fractions at different stages of the cell cycle based on their DNA content. Mock cells were used to set fluorescence background thresholds.

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2022-09-01
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