Targeted single cell RNA sequencing of T cells infiltrated in mouse gastric cancer.

YTN2 and YTN16 cells are chemically induced gastric cancer cell lines. YTN2 is spontaneously regressed in C57BL/6 mice in CD8+ T cell-dependent manner, while YTN16 grows progressively. To investigate tumor infiltrating T cells, we performed single-cell RNA-Seq of CD4+ and CD8+ T cells in YTN2 and YTN16 gastric tumor-bearing mice for 44 T cell-related genes (Supplementary Table 1). Mice were inoculated with 5 ×106 YTN2 or YTN16 on day 0. Tumors were harvested on day 7 and CD4+ and CD8+ T cells were sorted. Single-cell RNA-Seq was performed using vertical flow array chips (Shirai et al, Scientific Reports, 6, srep36014, 2016).