The proteomics effects of HSP60 chaperone deficiency in zebrafish larvae
收藏NIAID Data Ecosystem2026-05-02 收录
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https://www.omicsdi.org/dataset/pride/PXD048920
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资源简介：
To understand the effects of Hsp60 deficiency in developing vertebrates, we generated CRISPR/Cas9-mediated hspd1 knockout zebrafish lines by targeting exon 2 to induce a frameshift mutation. We selected an allele with a 56 base pair deletion inducing a frameshift mutation leading to loss of protein functions. We examined the proteome changes in zebrafish larvae at 5 days post fertilization (DPF). Wildtype control and hspd1-/- larvae at 5dpf, were analyzed by TMT and nanoLC-MS/MS based proteomcis. For this purpose, we studied five pools from each genotype, and each pool consisted of five larvae.
创建时间：
2024-09-27
